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Beijing TransGen Biotech easysee western blot kit
Easysee Western Blot Kit, supplied by Beijing TransGen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysee+western+blot+kit/easysee+western+blot+kit/pmc11439804__wjmh___42___865___s001-57-10-14
Average 90 stars, based on 1 article reviews
easysee western blot kit - by Bioz Stars, 2026-10
90/100 stars

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Western Blot:

Article Title: Upregulation of eIF2α by m 6 A modification accelerates the proliferation of pulmonary artery smooth muscle cells in MCT-induced pulmonary arterial hypertension rats.
Article Snippet: Pulmonary arterial hypertension (PAH) is a malignant cardiovascular disease.. Eukaryotic initiation factor 2α (eIF2α) plays an important role in the proliferation of pulmonary artery smooth muscle cells (PASMCs) in hypoxia-induced pulmonary hypertension (HPH) rats.. However, the regulatory mechanism of eIF2α remains poorly understood in PAH rats.

Article Title: Upregulation of AKAP12 by demethylation inhibits proliferation and increases chem osensitivity to adriamycin in leukemic cells
Article Snippet: Then, the membranes were incubated with the horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000) (Cat. no. 7074; Cell Signaling Technology, Inc) for 2 h at 37 °C. .. The Easysee Western Blot Kit (Beijing TransGen Biotech, Beijing, China) was utilized to measure the chemiluminescence signals. ..

Article Title: Ferroptosis Is Crucial for Cisplatin Induced Sertoli Cell Injury via N6-Methyladenosine Dependent Manner
Article Snippet: .. The signal bands were visualized with the use of an EasySee Western Blot kit (Beijing Transgen Biotech) and a chemiluminescence system (Bio-Rad). .. The band intensities were calculated by a software, ImageJ.

Article Title: The role of PKP1 in tumor progression in melanoma: Analysis of a cell adhesion-related model.
Article Snippet: The membranes were blocked with 5% skimmed milk and incubated overnight at 4 C with primary antibodies commercially purchased from Abcam, including PKP1 (ab183512), C-Myc (ab32072), CyclinD1 (ab16663), and CyclinD1 (ab219649). .. The membranes were washed and incubated with secondary antibodies for 1 h at 37 C. The proteins were visualized and photographed using a spectrophotometric system (Bio-Rad, CA, USA) and an EasySee Western Blot kit (Beijing Transgen Biotech, Beijing, China). ..

Article Title: Leveraging diverse cell-death related signature predicts the prognosis and immunotherapy response in renal clear cell carcinoma
Article Snippet: .. EasySee Western Blot kit (Beijing Transgen Biotech, Beijing, China) was used for detection following the experimental operation manual of manufacturer. .. Density measurements were carried out using ImageJ software (National Institute of Health, Bethesda, MD, USA), and the density of target protein bands were normalized with GAPDH.

Article Title: Baicalin Inhibits Cell Proliferation and Inflammatory Cytokines Induced by Tumor Necrosis Factor α (TNF-α) in Human Immortalized Keratinocytes (HaCaT) Human Keratinocytes by Inhibiting the STAT3/Nuclear Factor kappa B (NF-κB) Signaling Pathway
Article Snippet: Then, the membranes were incubated with the horseradish peroxidase (HRP)-conjugated secondary antibody (1: 2,000) (Cat. no. 7074; Cell Signaling Technology, Inc.) for 2 h at 37°C. .. The EasySee Western Blot Kit (Beijing TransGen Biotech, Beijing, China) was used to measure the chemiluminescence signals. ..

Article Title: ZNF471 Interacts with BANP to Reduce Tumour Malignancy by Inactivating PI3K/AKT/mTOR Signalling but is Frequently Silenced by Aberrant Promoter Methylation in Renal Cell Carcinoma
Article Snippet: Total protein from tissues or cells was obtained by lysis in RIPA lysis buffer containing 1% phenylmethylsulfonyl fluoride (PMSF), and protein concentrations were measured by a bicinchoninic acid (BCA) assay kit (Beyotime Institute of Biotechnology). .. Equal amounts of protein (30 μg/lane) were separated by electrophoresis in 10% SDS-polyacrylamide gels, followed by transfer to a PVDF membrane (0.2 μm), blocking of the membrane with 5% nonfat milk, incubation of the membrane with primary and secondary antibodies, and detection and image acquisition with an EasySee Western blot Kit (Beijing TransGen Biotech, Beijing, China) and a chemiluminescence system (Bio-Rad, CA, USA). .. Primary antibodies specific for the following proteins were used: ZNF471 (1:2000 dilution ratio) (HPA066695; Sigma-Aldrich), total AKT (1:1000 dilution ratio) (sc-8312; Santa Cruz Biotechnology), phospho-AKT (1:1000 dilution ratio) (sc-7985; Santa Cruz Biotechnology), PI3K (1:1000 dilution ratio) (4257; Cell Signaling Technology), phospho-PI3K (1:1000 dilution ratio) (4228; Cell Signaling Technology), mTOR (1:1000 dilution ratio) (2983; Cell Signaling Technology), phospho-mTOR (1:1000 dilution ratio) (5536; Cell Signaling Technology), E-cadherin (1:1000 dilution ratio) (ab40772; Abcam), N-cadherin (1:5000 dilution ratio) (ab76011; Abcam), vimentin (1:1000 dilution ratio) (ab20346; Abcam), snail (1:1000 dilution ratio) (ab216347; Abcam), PARP (1:1000 dilution ratio) (9542; Cell Signaling Technology), cleaved PARP (1:1000 dilution ratio) (5625; Cell Signaling Technology), caspase 3 (1:1000 dilution ratio) (9662; Cell Signaling Technology), cleaved caspase 3 (1:1000 dilution ratio) (9661; Cell Signaling Technology), caspase 8 (1:1000 dilution ratio) (4927; Cell Signaling Technology), cleaved caspase 8 (1:1000 dilution ratio) (8592; Cell Signaling Technology), p21 (1:1000 dilution ratio) (sc-126; Santa Cruz Biotechnology), p27 (1:1000 dilution ratio) (sc-393380; Santa Cruz Biotechnology), Cyclin D1 (1:1000 dilution ratio) (1677; Epitomics, Burlingame, CA), BANP (1:10000 dilution ratio) (ab72076; Abcam), and GAPDH (1:1000 dilution ratio) (5174S; Cell Signaling Technology).

Incubation:

Article Title: The role of PKP1 in tumor progression in melanoma: Analysis of a cell adhesion-related model.
Article Snippet: The membranes were blocked with 5% skimmed milk and incubated overnight at 4 C with primary antibodies commercially purchased from Abcam, including PKP1 (ab183512), C-Myc (ab32072), CyclinD1 (ab16663), and CyclinD1 (ab219649). .. The membranes were washed and incubated with secondary antibodies for 1 h at 37 C. The proteins were visualized and photographed using a spectrophotometric system (Bio-Rad, CA, USA) and an EasySee Western Blot kit (Beijing Transgen Biotech, Beijing, China). ..

Article Title: ZNF471 Interacts with BANP to Reduce Tumour Malignancy by Inactivating PI3K/AKT/mTOR Signalling but is Frequently Silenced by Aberrant Promoter Methylation in Renal Cell Carcinoma
Article Snippet: Total protein from tissues or cells was obtained by lysis in RIPA lysis buffer containing 1% phenylmethylsulfonyl fluoride (PMSF), and protein concentrations were measured by a bicinchoninic acid (BCA) assay kit (Beyotime Institute of Biotechnology). .. Equal amounts of protein (30 μg/lane) were separated by electrophoresis in 10% SDS-polyacrylamide gels, followed by transfer to a PVDF membrane (0.2 μm), blocking of the membrane with 5% nonfat milk, incubation of the membrane with primary and secondary antibodies, and detection and image acquisition with an EasySee Western blot Kit (Beijing TransGen Biotech, Beijing, China) and a chemiluminescence system (Bio-Rad, CA, USA). .. Primary antibodies specific for the following proteins were used: ZNF471 (1:2000 dilution ratio) (HPA066695; Sigma-Aldrich), total AKT (1:1000 dilution ratio) (sc-8312; Santa Cruz Biotechnology), phospho-AKT (1:1000 dilution ratio) (sc-7985; Santa Cruz Biotechnology), PI3K (1:1000 dilution ratio) (4257; Cell Signaling Technology), phospho-PI3K (1:1000 dilution ratio) (4228; Cell Signaling Technology), mTOR (1:1000 dilution ratio) (2983; Cell Signaling Technology), phospho-mTOR (1:1000 dilution ratio) (5536; Cell Signaling Technology), E-cadherin (1:1000 dilution ratio) (ab40772; Abcam), N-cadherin (1:5000 dilution ratio) (ab76011; Abcam), vimentin (1:1000 dilution ratio) (ab20346; Abcam), snail (1:1000 dilution ratio) (ab216347; Abcam), PARP (1:1000 dilution ratio) (9542; Cell Signaling Technology), cleaved PARP (1:1000 dilution ratio) (5625; Cell Signaling Technology), caspase 3 (1:1000 dilution ratio) (9662; Cell Signaling Technology), cleaved caspase 3 (1:1000 dilution ratio) (9661; Cell Signaling Technology), caspase 8 (1:1000 dilution ratio) (4927; Cell Signaling Technology), cleaved caspase 8 (1:1000 dilution ratio) (8592; Cell Signaling Technology), p21 (1:1000 dilution ratio) (sc-126; Santa Cruz Biotechnology), p27 (1:1000 dilution ratio) (sc-393380; Santa Cruz Biotechnology), Cyclin D1 (1:1000 dilution ratio) (1677; Epitomics, Burlingame, CA), BANP (1:10000 dilution ratio) (ab72076; Abcam), and GAPDH (1:1000 dilution ratio) (5174S; Cell Signaling Technology).

Electrophoresis:

Article Title: ZNF471 Interacts with BANP to Reduce Tumour Malignancy by Inactivating PI3K/AKT/mTOR Signalling but is Frequently Silenced by Aberrant Promoter Methylation in Renal Cell Carcinoma
Article Snippet: Total protein from tissues or cells was obtained by lysis in RIPA lysis buffer containing 1% phenylmethylsulfonyl fluoride (PMSF), and protein concentrations were measured by a bicinchoninic acid (BCA) assay kit (Beyotime Institute of Biotechnology). .. Equal amounts of protein (30 μg/lane) were separated by electrophoresis in 10% SDS-polyacrylamide gels, followed by transfer to a PVDF membrane (0.2 μm), blocking of the membrane with 5% nonfat milk, incubation of the membrane with primary and secondary antibodies, and detection and image acquisition with an EasySee Western blot Kit (Beijing TransGen Biotech, Beijing, China) and a chemiluminescence system (Bio-Rad, CA, USA). .. Primary antibodies specific for the following proteins were used: ZNF471 (1:2000 dilution ratio) (HPA066695; Sigma-Aldrich), total AKT (1:1000 dilution ratio) (sc-8312; Santa Cruz Biotechnology), phospho-AKT (1:1000 dilution ratio) (sc-7985; Santa Cruz Biotechnology), PI3K (1:1000 dilution ratio) (4257; Cell Signaling Technology), phospho-PI3K (1:1000 dilution ratio) (4228; Cell Signaling Technology), mTOR (1:1000 dilution ratio) (2983; Cell Signaling Technology), phospho-mTOR (1:1000 dilution ratio) (5536; Cell Signaling Technology), E-cadherin (1:1000 dilution ratio) (ab40772; Abcam), N-cadherin (1:5000 dilution ratio) (ab76011; Abcam), vimentin (1:1000 dilution ratio) (ab20346; Abcam), snail (1:1000 dilution ratio) (ab216347; Abcam), PARP (1:1000 dilution ratio) (9542; Cell Signaling Technology), cleaved PARP (1:1000 dilution ratio) (5625; Cell Signaling Technology), caspase 3 (1:1000 dilution ratio) (9662; Cell Signaling Technology), cleaved caspase 3 (1:1000 dilution ratio) (9661; Cell Signaling Technology), caspase 8 (1:1000 dilution ratio) (4927; Cell Signaling Technology), cleaved caspase 8 (1:1000 dilution ratio) (8592; Cell Signaling Technology), p21 (1:1000 dilution ratio) (sc-126; Santa Cruz Biotechnology), p27 (1:1000 dilution ratio) (sc-393380; Santa Cruz Biotechnology), Cyclin D1 (1:1000 dilution ratio) (1677; Epitomics, Burlingame, CA), BANP (1:10000 dilution ratio) (ab72076; Abcam), and GAPDH (1:1000 dilution ratio) (5174S; Cell Signaling Technology).

Membrane:

Article Title: ZNF471 Interacts with BANP to Reduce Tumour Malignancy by Inactivating PI3K/AKT/mTOR Signalling but is Frequently Silenced by Aberrant Promoter Methylation in Renal Cell Carcinoma
Article Snippet: Total protein from tissues or cells was obtained by lysis in RIPA lysis buffer containing 1% phenylmethylsulfonyl fluoride (PMSF), and protein concentrations were measured by a bicinchoninic acid (BCA) assay kit (Beyotime Institute of Biotechnology). .. Equal amounts of protein (30 μg/lane) were separated by electrophoresis in 10% SDS-polyacrylamide gels, followed by transfer to a PVDF membrane (0.2 μm), blocking of the membrane with 5% nonfat milk, incubation of the membrane with primary and secondary antibodies, and detection and image acquisition with an EasySee Western blot Kit (Beijing TransGen Biotech, Beijing, China) and a chemiluminescence system (Bio-Rad, CA, USA). .. Primary antibodies specific for the following proteins were used: ZNF471 (1:2000 dilution ratio) (HPA066695; Sigma-Aldrich), total AKT (1:1000 dilution ratio) (sc-8312; Santa Cruz Biotechnology), phospho-AKT (1:1000 dilution ratio) (sc-7985; Santa Cruz Biotechnology), PI3K (1:1000 dilution ratio) (4257; Cell Signaling Technology), phospho-PI3K (1:1000 dilution ratio) (4228; Cell Signaling Technology), mTOR (1:1000 dilution ratio) (2983; Cell Signaling Technology), phospho-mTOR (1:1000 dilution ratio) (5536; Cell Signaling Technology), E-cadherin (1:1000 dilution ratio) (ab40772; Abcam), N-cadherin (1:5000 dilution ratio) (ab76011; Abcam), vimentin (1:1000 dilution ratio) (ab20346; Abcam), snail (1:1000 dilution ratio) (ab216347; Abcam), PARP (1:1000 dilution ratio) (9542; Cell Signaling Technology), cleaved PARP (1:1000 dilution ratio) (5625; Cell Signaling Technology), caspase 3 (1:1000 dilution ratio) (9662; Cell Signaling Technology), cleaved caspase 3 (1:1000 dilution ratio) (9661; Cell Signaling Technology), caspase 8 (1:1000 dilution ratio) (4927; Cell Signaling Technology), cleaved caspase 8 (1:1000 dilution ratio) (8592; Cell Signaling Technology), p21 (1:1000 dilution ratio) (sc-126; Santa Cruz Biotechnology), p27 (1:1000 dilution ratio) (sc-393380; Santa Cruz Biotechnology), Cyclin D1 (1:1000 dilution ratio) (1677; Epitomics, Burlingame, CA), BANP (1:10000 dilution ratio) (ab72076; Abcam), and GAPDH (1:1000 dilution ratio) (5174S; Cell Signaling Technology).

Blocking Assay:

Article Title: ZNF471 Interacts with BANP to Reduce Tumour Malignancy by Inactivating PI3K/AKT/mTOR Signalling but is Frequently Silenced by Aberrant Promoter Methylation in Renal Cell Carcinoma
Article Snippet: Total protein from tissues or cells was obtained by lysis in RIPA lysis buffer containing 1% phenylmethylsulfonyl fluoride (PMSF), and protein concentrations were measured by a bicinchoninic acid (BCA) assay kit (Beyotime Institute of Biotechnology). .. Equal amounts of protein (30 μg/lane) were separated by electrophoresis in 10% SDS-polyacrylamide gels, followed by transfer to a PVDF membrane (0.2 μm), blocking of the membrane with 5% nonfat milk, incubation of the membrane with primary and secondary antibodies, and detection and image acquisition with an EasySee Western blot Kit (Beijing TransGen Biotech, Beijing, China) and a chemiluminescence system (Bio-Rad, CA, USA). .. Primary antibodies specific for the following proteins were used: ZNF471 (1:2000 dilution ratio) (HPA066695; Sigma-Aldrich), total AKT (1:1000 dilution ratio) (sc-8312; Santa Cruz Biotechnology), phospho-AKT (1:1000 dilution ratio) (sc-7985; Santa Cruz Biotechnology), PI3K (1:1000 dilution ratio) (4257; Cell Signaling Technology), phospho-PI3K (1:1000 dilution ratio) (4228; Cell Signaling Technology), mTOR (1:1000 dilution ratio) (2983; Cell Signaling Technology), phospho-mTOR (1:1000 dilution ratio) (5536; Cell Signaling Technology), E-cadherin (1:1000 dilution ratio) (ab40772; Abcam), N-cadherin (1:5000 dilution ratio) (ab76011; Abcam), vimentin (1:1000 dilution ratio) (ab20346; Abcam), snail (1:1000 dilution ratio) (ab216347; Abcam), PARP (1:1000 dilution ratio) (9542; Cell Signaling Technology), cleaved PARP (1:1000 dilution ratio) (5625; Cell Signaling Technology), caspase 3 (1:1000 dilution ratio) (9662; Cell Signaling Technology), cleaved caspase 3 (1:1000 dilution ratio) (9661; Cell Signaling Technology), caspase 8 (1:1000 dilution ratio) (4927; Cell Signaling Technology), cleaved caspase 8 (1:1000 dilution ratio) (8592; Cell Signaling Technology), p21 (1:1000 dilution ratio) (sc-126; Santa Cruz Biotechnology), p27 (1:1000 dilution ratio) (sc-393380; Santa Cruz Biotechnology), Cyclin D1 (1:1000 dilution ratio) (1677; Epitomics, Burlingame, CA), BANP (1:10000 dilution ratio) (ab72076; Abcam), and GAPDH (1:1000 dilution ratio) (5174S; Cell Signaling Technology).



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